Thermo Fisher Scientific Phospho-CDK4 (Thr172) Recombinant Rabbit Monoclonal Antibody (9H2L7)

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 2 µg/mL

Product Specifications

Product Specific Information

This antibody is predicted to react with Monkey, Sheep, Rat, and Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone specific antibody DNA sequences from immunoreactive rabbits, followed by screening of individual clones to select optimal candidates for production.
Advantages include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire

Target Information

CDK4/cyclin D3 serine/threonine kinase is a dimeric protein complex. CDK4, a member of the cyclin-dependent kinase family, regulates cell proliferation during the G1 phase of the cell cycle.
It forms a complex with D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 mediates phosphorylation of the C-terminal region of Rb protein, leading to active transcriptional repression of the E2F complex.
CDC37 and HSP90 preferentially associate with CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of G1 cyclin-dependent kinases CDK4 and CDK2.
Although CDK6 and CDK4 both phosphorylate multiple residues in the Rb protein, they exhibit different residue selectivity in vitro: CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.