
Thermo Fisher Scientific SERCA1 Polyclonal Antibody
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SERCA1 단백질을 표적하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체입니다. Western blot, IHC, ICC/IF에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 동결 건조 형태로 제공되며 -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:200 | |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | 1:400 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)TPDQVKRHLEKYG, corresponding to amino acid residues 25–37 of rat SERCA1, Cytoplasmic, N-terminus |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution: Add 50 µL or 0.2 mL double distilled water (DDW), depending on sample size.
- The antibody ships as a lyophilized powder at room temperature.
- Upon arrival, store at -20°C.
- Reconstituted solution can be stored at 4°C for up to 1 week.
- For long-term storage, aliquot and freeze at -20°C.
- Avoid multiple freeze/thaw cycles.
- Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
ATP-dependent calcium pumps maintain low cytoplasmic calcium concentrations. SERCA ATPases are encoded by a family of related enzymes located in intracellular organelles.
- SERCA1: Expressed exclusively in type II (fast) skeletal muscle.
- SERCA2: Generates tissue-dependent isoforms—SERCA2a in type I (slow) skeletal, cardiac, and smooth muscle; SERCA2b in all cell types.
- SERCA3: Found in non-muscle cells, less characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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