Thermo Fisher Scientific PMCA1 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:200

Immunohistochemistry (Frozen) (IHC (F))

• Tested Dilution: 1:300

Product Specifications

Product Specific Information

• Reconstitution: 25 µL, 50 µL, or 0.2 mL double distilled water (DDW), depending on sample size.
• Ships as lyophilized powder at room temperature; store at -20°C upon arrival.
• Reconstituted solution can be stored at 4°C for up to 1 week.
• For long-term storage, aliquot and store at -20°C.
• Avoid multiple freezing and thawing cycles.
• Centrifuge all antibody preparations before use (10,000 × g, 5 min).

Target Information

The calcium pump of the plasma membrane, termed PMCA ATPase, transports calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to other ATPases such as SERCA ATPase and the sodium/potassium pump.
There are four genes encoding PMCA ATPase, and alternative splicing generates approximately 20 isoforms.
mRNA distribution studies show that gene products 1 and 4 are expressed in most tissues, while 2 and 3 are more tissue-specific.
In the pancreas, beta cells express only the 4b isoform, whereas alpha and gamma cells express both 4a and 4b isoforms.
Different splice variants exhibit distinct affinities for calcium and calmodulin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.