Thermo Fisher Scientific P21 Polyclonal Antibody
다양한 종(Human, Mouse, Rat, Sheep)에 반응하는 Rabbit Polyclonal Anti-P21 항체입니다. Western blot, IHC, ICC, Flow cytometry, ELISA 등 다양한 응용에 적합합니다. Protein A로 정제되었으며, -20°C에서 보관합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Sheep, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human P21, amino acids 21–100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Protein p21 is a tumor suppressor protein that inhibits Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. It plays a key role in the cellular response to genotoxic stress and is a major transcriptional target of p53. Nuclear p21 binds and inhibits CDK1 and CDK2, blocking cell cycle progression from G1 to S phase or G2 to mitosis after DNA damage, allowing DNA repair. In the cytoplasm, p21 has anti-apoptotic activity by inhibiting caspase 3 and the apoptotic kinases ASK1 and JNK. It exhibits dual roles in carcinogenesis, functioning both as a tumor suppressor and as an oncogene depending on context.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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