Thermo Fisher Scientific AFAP1L2 Monoclonal Antibody (4E12D7)
AFAP1L2 단백질을 인식하는 Mouse monoclonal antibody(4E12D7). WB, Flow Cytometry, ELISA에 사용 가능. Human 시료 반응성. Protein G 정제, PBS buffer에 0.05% sodium azide 함유. 단기 4°C, 장기 -20°C 보관.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific AFAP1L2 Monoclonal Antibody (4E12D7)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4E12D7 |
| Immunogen | Purified recombinant fragment of human AFAP1L2 (AA: 674–818) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787239 |
Product Specific Information
MA5-31615 has been tested in indirect ELISA.
Target Information
AFAP1L2, also known as XB130, is structurally similar to actin-filament-associated protein (AFAP). It contains multiple SH2- and SH3-binding motifs, two pleckstrin homology domains, a coiled-coil region, and several phosphorylation sites. AFAP1L2 interacts with and is phosphorylated by c-Src tyrosine kinase. Knockdown of AFAP1L2 via siRNA reduces Src activity, IL-8 production, and EGF-induced phosphorylation of Akt and GSK3β, affecting cell cycle regulation in human lung epithelial cells. AFAP1L2 is highly expressed in thyroid tissue and serves as a substrate for RET/PTC kinase, implicated in papillary thyroid cancer. Down-regulation of AFAP1L2 reduces Akt activity, inhibiting cancer cell proliferation and survival, suggesting its potential as a therapeutic target. At least four isoforms of AFAP1L2 are known.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.