Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Brilliant Ultra Violet 615, eBioscience™

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 661, 737, 805
• Brilliant Violet 421, 480, 605, 650, 711
• eFluor 450, 506
• FITC
• NovaFluor Blue, Red, Yellow series
• PE, PE-Cyanine5, 5.5, 7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The HI30 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells except circulating erythrocytes and platelets. The cytoplasmic domain has tyrosine phosphatase activity and plays a key role in lymphocyte activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for use at 5 µL/test (0.25 µg)
• Recommended Cell Count: 10⁵–10⁸ cells/test

Fluorochrome Information:
Brilliant Ultra Violet™ 615 (BUV615) emits at 615 nm and is designed for cytometers equipped with a 355 nm UV laser.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Light Sensitivity:
Protect from light to prevent photo-induced oxidation.

Fixation:
Samples can be stored in IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54) for up to 3 days at 4°C in the dark.

Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet (UV) Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, Leukocyte Common Antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, constituting about 10% of surface proteins in lymphocytes. It is absent on non-hematopoietic cells and tissues. CD45 regulates T- and B-cell receptor signaling, JAK kinase activity, and integrin-mediated survival pathways. Multiple isoforms (e.g., CD45RA) arise from alternative splicing and differ in expression and membrane localization depending on cell type and physiological state.

For Research Use Only. Not for use in diagnostic procedures.

Excitation/Emission Spectra

350 / 607 nm
View spectra

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