Thermo Fisher Scientific CD15 Monoclonal Antibody (MMA(Leu-M1))
CD15 (Leu-M1) 단클론 항체로 인간 시료에 반응하며, IHC, ICC/IF, Flow Cytometry에 적합. U-937 세포주로부터 유래한 면역원 사용. 액상 형태, 50 µg/mL 농도, PBS 버퍼에 0.1% BSA 및 0.09% sodium azide 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µL / 10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MMA (Leu-M1) |
| Immunogen | U-937 histiocytic cell line |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 50 µg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins, and proteoglycans in various cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa, nervous system, and tumor cells.
Variants include sialyl-Lewis x and sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation via calcium-dependent Le(x)-Le(x) interaction, involved in processes like embryo compaction and autoaggregation of teratocarcinoma cells.
Sialyl-Le(x) and its isomer sialyl-Le(a) serve as selectin ligands. CD15 expression is widely used to confirm diagnosis of Hodgkin’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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