Thermo Scientific™

T4 RNA Ligase (10 U/μL)

Thermo Scientific T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5-phosphate and 3-hydroxyl termini자세히 알아보기

Thermo Scientific T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5-phosphate and 3-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.

The minimal substrate is a nucleoside 3,5-biphosphate in intermolecular reaction and oligonucleotide of 8bases in intramolecular reaction.

Applications

• RNA 3-end labeling with cytidine 3,5-bis \[alpha-32P\] phosphate • Joining RNA to RNA • Synthesis of oligoribonucleotides and oligodeoxyribonucleotides • Specific modifications of tRNAs • Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5 RACE (Rapid Amplification of cDNA Ends)
• Site-specific generation of composite primers for PCR

Note

The recommended BSA concentration in the reaction mixture is 0.1mg/mL.

사양

농도10 U/μL

효소T4 RNA Ligase

함께 사용가능한 버퍼10X Reaction Buffer

수량1,000 units

제품 유형T4 RNA Ligase

Unit SizeEach