Thermo Fisher Scientific Cathepsin F Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000
• Recommended positive controls: A431, H1299, HeLa, HepG2
• Predicted reactivity: Mouse (84%), Rat (84%), Bovine (84%)

Product Specifications

Product Specific Information

• Store product as a concentrated solution.
• Centrifuge briefly prior to opening the vial.

Target Information

Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region.

The very long (251 amino acid residues) proregion of the cathepsin F precursor contains:

• A C-terminal domain similar to the pro-segment of cathepsin L-like enzymes
• A 50-residue flexible linker peptide
• An N-terminal domain predicted to adopt a cystatin-like fold

The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains elements known to be important for inhibitory activity.

CTSF encodes a predicted protein of 484 amino acids containing a 19 residue signal peptide. Cathepsin F includes five potential N-glycosylation sites and may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed and maps to chromosome 11q13, near the gene encoding cathepsin W.

⚠ WARNING
This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.