Thermo Fisher Scientific GAPDH Polyclonal Antibody
GAPDH 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, Western blot에 적합합니다. 인간, 마우스, 원숭이, 랫트에 반응하며, glycolysis 관련 연구 및 로딩 컨트롤용으로 활용됩니다. Lyophilized 형태로 4°C 또는 -20°C 보관 가능합니다.
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Applications
- Western Blot (WB): Tested dilution 1:1,000
- Miscellaneous PubMed (Misc): 1 publication available
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from rat GAPDH conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
- The antigen is homologous in mouse and shares 90% identity with human sequence.
- Specificity: GAPDH.
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis.
It exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH is ubiquitously expressed and participates in various cellular processes such as:
- DNA replication and repair
- Nuclear RNA export
- Membrane fusion and microtubule bundling
- Apoptosis and neurodegenerative disease mechanisms (e.g., Alzheimer’s and Huntington’s disease)
Due to its high expression in most tissues, GAPDH is widely used as a protein loading control in Western Blot analysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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