Thermo Fisher Scientific Bcl-6 Monoclonal Antibody (BCL6, 1982)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Peptide Array (Array)

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

• Tested in direct ELISA (use antibody at 2–4 µg/mL for coating).
• For immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

Bcl-6 proto-oncogene product is a Kruppel-type zinc-finger protein expressed mainly in normal germinal center B cells and related lymphomas.
It is involved in chromosome rearrangements at 3q27 in non-Hodgkin’s lymphomas, with Bcl-6 rearrangements detected in 33–45% of diffuse large B cell lymphomas.
Immunohistochemical detection of Bcl-6 has been reported in follicular lymphomas, diffuse large B cell lymphomas, Burkitt’s lymphomas, and nodular lymphocyte predominant Hodgkin’s disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.