Thermo Fisher Scientific SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eFluor 570, eBioscience
SSEA-4 항원을 인식하는 마우스 단클론 항체로, 인간 줄기세포 및 유도만능줄기세포(iPS)에서 발현을 검출합니다. eFluor 570 형광 표지로 면역세포염색(ICC/IF)에 적합하며, 555/570 nm 파장에서 최적의 형광을 제공합니다. 4°C 암소 보관, 동결 금지.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMC-813-70 (MC-813-70) |
| Conjugate | eFluor™ 570 |
| Excitation / Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11217876 |
Additional Formats
Product Specific Information
The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein.
Expression is found on human teratocarcinoma stem cells (EC), embryonic germ cells (EG), induced pluripotent stem (iPS) cells, and embryonic stem cells (ES), with decreased expression upon differentiation. Expression has also been observed on bone marrow mesenchymal stem cells.
This antibody crossreacts with mouse, rabbit, canine, chicken, and Old/New World monkeys.
Applications Reported: Suitable for immunocytochemistry (ICC/IF).
Applications Tested: Validated on fixed NTERA-2 and 2102Ep cells at ≤10 µg/mL. Optimize dilution for best performance.
Filter Recommendation:
For eFluor® 570 conjugate, use filters capturing 570 nm emission (e.g., Excitation 545/25, 565LP, Emission 605/70).
Standard Alexa Fluor® 555 or TRITC filters are acceptable.
Excitation: 555 nm
Emission: 570 nm
Target Information
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen expressed with SSEA-3, TRA-1-60, and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS).
In human cells, these markers are downregulated during differentiation, while SSEA-1 is absent in undifferentiated cells and upregulated after retinoic acid-mediated differentiation.
In mouse stem cells, SSEA-1 expression decreases with differentiation, whereas SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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