Thermo Fisher Scientific SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eFluor 570, eBioscience

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

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Immunocytochemistry (ICC/IF)

10 µg/mL

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Product Specifications

Published species

Not Applicable

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

eBioMC-813-70 (MC-813-70)

Conjugate

eFluor™ 570 eFluor™ 570 eFluor™ 570

View additional formats

• Unconjugated
• Alexa Fluor 488
• Biotin
• eFluor 660
• PE
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

556/569 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11217876

Product Specific Information

Description: The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), human induced pluripotent stem (iPS) cells, and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.

The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.

Applications Reported: This eBioMC-813-70 (MC-813-70) antibody has been reported for use in immunocytochemistry. This product has not been validated for flow cytometric analysis.

Applications Tested: This eBioMC-813-70 (MC-813-70) antibody has been tested by immunocytochemistry on fixed NTERA-2 and 2102Ep cells and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filter Recommendation: When using this eFluor® 570 antibody conjugate, we recommend a filter that will capture the 570 emission wavelength (for example, Excitation 545/25, 565LP, Emission 605/70). A standard Alexa Fluor® 555 or TRITC filter is acceptable.

Excitation: 555 nm; Emission: 570 nm

Target Information

SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen, expressed along with SSEA-3, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA-1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.