Thermo Fisher Scientific ERK1/2 Monoclonal Antibody (5AD13MA), eFluor 570, eBioscience
ERK1/2 단백질을 인식하는 마우스 모노클로날 항체로, eFluor 570 형광 표지. 세포 및 조직 수준의 ERK1/2 발현 분석에 적합하며, WB, IHC, ICC 등 다양한 응용 가능. 556/569 nm 형광 특성, 4°C 보관, 암조건 유지 필요.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | – | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | – |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 570, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5AD13MA |
| Conjugate | eFluor™ 570 |
| Excitation / Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2573620 |
Additional Formats
Product Specific Information
The 5AD13MA monoclonal antibody recognizes human, mouse, and rat ERK1/2 (p44/42, MAPK3/1). ERK1/2 are conserved serine/threonine kinases involved in cell proliferation, differentiation, motility, and survival. They are activated by MEK1/2 via phosphorylation and regulate downstream targets such as p90RSK and Elk-1. ERK signaling is implicated in many cancers, making it an important diagnostic and therapeutic target.
Applications Reported:
Suitable for immunohistochemistry (paraffin-embedded sections), microscopy, and immunocytochemistry.
Applications Tested:
Validated for immunocytochemistry of methanol-fixed cells at ≤ 5 µg/mL. Optimal titration is recommended for specific assays.
Filter Recommendation:
Use a filter capturing 570 nm emission (e.g., Excitation 545/25, 565LP; Emission 605/70). Standard Alexa Fluor™ 555 or TRITC filters are acceptable.
Excitation / Emission: 555 nm / 570 nm
Target Information
ERK1 and ERK2 are key regulators of meiosis, mitosis, and postmitotic functions. They are activated by various stimuli including growth factors, cytokines, and GPCR ligands. Upon activation, ERKs phosphorylate cytoplasmic targets or translocate to the nucleus to activate transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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