Thermo Scientific™

Uracil-DNA Glycosylase (1 U/μL)

Thermo Scientific Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic자세히 알아보기

Thermo Scientific Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA (see Figure 1 in Supporting Data). The enzyme shows no activity on RNA.

Highlights

• Active in Thermo Scientific buffers for restriction enzymes and thermophilic polymerases

Applications

• Control of carry-over contamination in PCR
• Glycosylase mediated single nucleotide polymorphism detection (GMPD)
• Site-directed mutagenesis
• As a probe for protein-DNA interaction studies
• SNP genotyping
• Cloning of PCR products
• Generation of single strand overhangs of PCR products and cDNA

Note

The abasic sites formed in DNA by Uracil-DNA Glycosylase may be subsequently cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. UDG (UNG) is active in the presence or absence of divalent cations.

Use of this enzyme in certain applications may be covered by patents and may require a license.

사양

농도1 U/μL

효소Uracil-DNA Glycosylase

함께 사용가능한 버퍼10X Reaction Buffer

수량200 units

제품 유형Uracil DNA Glycosylase

Unit SizeEach