Thermo Fisher Scientific GSDMD Recombinant Rabbit Monoclonal Antibody (8X4F4)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8X4F4

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 346-484 of human GSDMD (NP_079012.3). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GSDMD,uniProtId:P57764-1,ncbiNodeId:9606,antigenRange:346-484,antigenLength:484,antigenImageFileName:MA5-51265_GSDMD_P57764-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51265_GSDMD_P57764-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_3093761

Product Specific Information

Immunogen sequence: DGPAGAVLEC LVLSSGMLVP ELAIPVVYLL GALTMLSETQ HKLLAEALES QTLLGPLELV GSLLEQSAPW QERSTMSLPP GLLGNSWGEG APAWVLLDEC GLELGEDTPH VCWEPQAQGR MCALYASLAL LSGLSQEPH

Target Information

GSDMD (gasdermin-D) promotes pyroptosis in response to microbial infection and danger signals. It is produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as, non-canonical (such as cytosolic LPS) inflammasome activators. After cleavage, GSDMD moves to the plasma membrane where it strongly binds to inner leaflet lipids including phosphatidylinositol 4-phosphate, phosphatidylinositol (4,5)-biphosphate, phosphatidylinositol (3,4,5)-biphosphate, phosphatidic acid, and phosphatidylserine. GSDMD also exhibits bactericidal activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.