Thermo Fisher Scientific NMT2 Monoclonal Antibody (OTI2A5), TrueMAB

Thermo Fisher Scientific NMT2 Monoclonal Antibody (OTI2A5), TrueMAB

Applications and Tested Dilution

• Western Blot (WB): 1:500–1:2,000
• Immunohistochemistry (Paraffin) (IHC (P)): 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

Proteolytic degradation is essential for maintaining proper levels of short-lived and regulatory proteins involved in cellular metabolism, stress response, antigen presentation, receptor modulation, ion channel regulation, cell cycle, transcription, and signaling.
The ubiquitin-proteasome pathway is responsible for degrading most cytosolic and nuclear proteins, while others are processed via the vacuolar pathway (endosomes, lysosomes, ER).
The 26S proteasome is an ATP-dependent, multisubunit complex (~2.5 MDa) composed of a 20S core and 19S regulatory subunits that recognize ubiquitinated proteins and facilitate their degradation.
Defects in this pathway are linked to diseases such as cystic fibrosis, Angelman’s syndrome, and Liddle syndrome.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.