
Thermo Fisher Scientific IGJ Monoclonal Antibody (OTI2B1), TrueMAB
IGJ 단백질을 인식하는 마우스 단클론 항체로, Western Blot, IHC, Flow Cytometry에 적합. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용 가능. 인체 시료 반응성이 높으며, 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2B1 |
| Immunogen | Full length human recombinant protein of human IGJ produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
J chain is a small glycopeptide synthesized by plasma cells secreting polymeric immunoglobulins. It is linked to IgA and IgM via disulfide bonds, and can also be detected in IgG- and IgD-containing cells. J chains are abundant in immunoglobulin-positive cells within germinal centers of tonsil and lymph node. Expression occurs early in B cell differentiation and persists in cells producing IgA or IgM.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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