Thermo Fisher Scientific ZO-1 Polyclonal Antibody
ZO-1 단백질을 인식하는 염소 폴리클로날 항체로, IHC 및 ICC/IF에 사용 가능. 인간 및 마우스 반응성 확인됨. 합성 펩타이드 면역원 기반, 액상 형태로 제공. 연구용으로 세포 접합 및 신호전달 연구에 적합.
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Applications
Immunohistochemistry (IHC)
- Tested Dilution: Not specified
- View 3 publications
Immunocytochemistry (ICC/IF)
- Tested Dilution: 10 µg/mL
- View 3 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (PKPTSQNQFSEHDKT) corresponding to the internal amino acids of TJP1 (aa 1308-1323) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10988668 |
Product Specific Information
- Tested in Peptide ELISA: antibody detection limit dilution 8,000.
Target Information
ZO-1 forms complexes with either ZO-2 or ZO-3. These proteins associate with claudin, occludin, and F-actin at tight junction strands, linking the actin cytoskeleton and tight junctions.
ZO-1 expression is significantly reduced in many breast cancer lines.
ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, while ZO-1 is also expressed at cardiac myocyte junctions.
ZO-1 may be involved in signal transduction at cell-cell junctions.
Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene.
Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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