Thermo Fisher Scientific ATP5A1 Monoclonal Antibody (15H4C4)
ATP5A1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 적합합니다. 마우스 유래 IgG2b 항체이며, 미토콘드리아 ATP 합성효소의 알파 서브유닛을 표적합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1.0 µg/mL | View 15 publications |
| Immunohistochemistry (IHC) | 1 µg/mL | View 4 publications |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL | View 2 publications |
| Flow Cytometry (Flow) | 1 µg/mL | - |
| Immunoprecipitation (IP) | - | View 1 publication |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Fruit fly, Human, Mouse, Rat |
| Published Species | C. elegans, Fruit fly, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 15H4C4 |
| Immunogen | Bovine Complex V |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | HEPES buffered saline |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2533548 |
Product Specific Information
- For IHC, use heat-mediated antigen retrieval with citrate buffer (pH 6) before staining.
- Positive control: Human, bovine, mouse, and rat heart mitochondria.
Target Information
This gene encodes a subunit of mitochondrial ATP synthase, which catalyzes ATP synthesis using an electrochemical proton gradient across the inner membrane during oxidative phosphorylation.
ATP synthase consists of two linked multi-subunit complexes:
- F1: Soluble catalytic core composed of subunits α, β, γ, δ, and ε (stoichiometry 3α:3β:1γ:1δ:1ε)
- Fo: Membrane-spanning proton channel composed of subunits a, b, and c
This gene encodes the α subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. Pseudogenes are located on chromosomes 9, 2, and 16.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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