Thermo Fisher Scientific ATP5A1 Monoclonal Antibody (15H4C4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

View 15 publications 15 publications

Immunohistochemistry (IHC)

1 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 2 publications 2 publications

Flow Cytometry (Flow)

1 µg/mL

-

Immunoprecipitation (IP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Fruit fly, Human, Mouse, Rat

Published species

C. elegans, Fruit fly, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

15H4C4

Immunogen

Bovine Complex V

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2533548

Product Specific Information

When performing IHC use heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Positive control: Human, bovine, mouse and rat heart mitochondria.

Target Information

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.