Thermo Fisher Scientific SWAP70 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000-1:20,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SWAP70. The exact sequence is proprietary. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SWAP70,uniProtId:Q9UH65-1,ncbiNodeId:9606,antigenRange:585,antigenLength:585,antigenImageFileName:PA5-78432_SWAP70_Q9UH65-1_Rabbit.svg,antigenImageFileNamePDP:PA5-78432_SWAP70_Q9UH65-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2736669

Product Specific Information

Positive Control: Jurkat, Raji, NCI-H929, 293T, A431, HeLa, HepG2

Predicted Reactivity: Rat (95%), Chicken (89%), Rhesus Monkey (99%), Bovine (93%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

SWAP70 is a 585 amino acid regulatory protein belonging to the pleckstrin-like family with a PH domain essential for phosphatidylinositol 3, 4, 5-trisphosphate binding. SWAP70 acts as a link between the recognition of specific switch regions and causation of a DNA break and is involved in both nuclear events and in signaling B-cell activation. As a guanine nucleotide exchange factor (GEF) it transduces signals from tyrosine kinase receptors to RAC, independent of RAS. As a multiple functional signaling protein, and a component of SWAP enzyme complex, it mediates signaling of membrane ruffling by tyrosine kinase growth factor receptors, actin cytoskeleton and cell protrusion. It is widely expressed in most tissues, mature B-cells, including those associated with mucosa-associated tissue and bronchus-associated tissue, monocytes and macrophages.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.