Thermo Fisher Scientific Phospho-MYL12A (Ser1) Polyclonal Antibody
Phospho-MYL12A (Ser1) 폴리클로날 항체로 Western blot 및 면역세포염색에 적합. Rabbit IgG 기반으로 다양한 종(인간, 마우스, 랫, 소)에 반응. 항원 친화 크로마토그래피로 정제되었으며, -20°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Phospho-MLC (Ser-1) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1 in bovine myosin regulatory light chain 9 (Myl9). This peptide sequence is highly conserved in human, rat, and mouse Myl9, and in other smooth muscle and non-muscle MLCs. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942954 |
Product Specific Information
This antibody detects a 20 kDa band corresponding to the molecular weight of myosin light chain in human A431 treated with EGF or calyculin A. It also detects the 20 kDa myosin light chain in mouse C2C12, rat PC12, and rabbit fibroblasts treated with calyculin A.
Target Information
This gene encodes a nonsarcomeric myosin regulatory light chain. The protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. It may also participate in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1, which represses p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is located on chromosome 8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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