Thermo Fisher Scientific Nucleolin Polyclonal Antibody
Western blot, IHC, ICC 등 다양한 응용에 적합한 Nucleolin 폴리클로날 항체. 사람, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 고품질 항체. 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1 µg/mL |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:400 |
| Immunocytochemistry (ICC/IF) | 1–5 µg/mL |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 1–100 of Human Nucleolin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10981301 |
Product Specific Information
- Heat-mediated antigen retrieval is recommended prior to tissue staining.
- Store antibody at 4°C for 1–2 weeks. For long-term storage, store at -20°C.
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in the synthesis and maturation of ribosomes. It is primarily located in the dense fibrillar regions of the nucleolus.
The human NCL gene consists of 14 exons and 13 introns, spanning approximately 11 kb. Intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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