Thermo Fisher Scientific POU6F1 Monoclonal Antibody (5F9)
POU6F1 단백질을 인식하는 Mouse monoclonal antibody. Western blot과 ELISA에 사용 가능. 높은 특이성과 재현성을 제공하며, 연구용으로만 사용. -20°C에서 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| ELISA | 10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5F9 |
| Immunogen | POU6F1 (NP_002693, 193 a.a. ~ 301 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 kDa. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
ITPKSAQKLK PVLEKWLNEA ELRNQEGQQN LMEFVGGEPS KKRKRRTSFT PQAIEALNAY FEKNPLPTGQ EITEIAKELN YDREVVRVWF CNRRQTLKNT SKLNVFQIP
Target Information
Brn-5 (Brain-5), also known as POU6F1, MPOU or TCFB1, is a widely expressed protein but during embryogenesis is exclusively found in the developing brain and spinal cord. As is characteristic of Brn family members, Brn-5 contains two DNA-binding domains, namely the POU-specific domain and the POU homeodomain, which each contain an HTH (helix-turn-helix) motif. Brn-5 binds to CRH (corticotrophin-releasing hormone) elements with high affinity and is capable of both enhancing Prolactin gene expression and activating Pit-1 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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