Thermo Fisher Scientific GCLM Polyclonal Antibody
Thermo Fisher Scientific의 GCLM Polyclonal Antibody는 인간 및 마우스 시료에 반응하는 Rabbit IgG 항체로, Western blot과 ELISA에 적합합니다. 재조합 GCLM 단백질(aa 1-274)을 면역원으로 사용하며, 고순도 Affinity Chromatography로 정제된 액상 형태입니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
ELISA
- Tested Dilution: 1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–274 of human GCLM (NP_002052.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.57 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913818 |
Product Specific Information
- Positive test controls include: H460, HT-29, K562, ES2, U-937, 22Rv1, U-251MG, mouse liver.
Immunogen sequence:
MGTDSRAAKA LLARARTLHL QTGNLLNWGR LRKKCPSTHS EELHDCIQKT LNEWSSQINP DLVREFPDVL ECTVSHAVEK INPDEREEMK VSAKLFIVES NSSSSTRSAV DMACSVLGVA QLDSVIIASP PIEDGVNLSL EHLQPYWEEL ENLVQSKKIV AIGTSDLDKT QLEQLYQWAQ VKPNSNQVNL ASCCVMPPDL TAFAKQFDIQ LLTHNDPKEL LSEASFQEAL QESIPDIQAH EWVPLWLLRY SVIVKSRGII KSKGYILQAK RRGS
Target Information
Gamma-glutamylcysteine synthetase (gamma-GCS) is the rate-limiting enzyme for glutathione (GSH) synthesis.
GSH acts as a vital intra- and extracellular antioxidant in mammalian cells.
Gamma-GCS is a heterodimer composed of a heavy catalytic subunit and a light regulatory subunit, responsive to inflammation, phenolic antioxidants, heat shock, oxidants, and cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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