Thermo Fisher Scientific ARHGEF7 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ARHGEF7. Recombinant protein control fragment (Product #RP-88620). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ARHGEF7,uniProtId:Q14155-4,ncbiNodeId:9606,antigenRange:516-650,antigenLength:803,antigenImageFileName:PA5-52097_ARHGEF7_Q14155-4_Rabbit.svg,antigenImageFileNamePDP:PA5-52097_ARHGEF7_Q14155-4_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2638181

Product Specific Information

Immunogen sequence: RMSGFIYQGK LPTTGMTITK LEDSENHRNA FEISGSMIER ILVSCNNQQD LQEWVEHLQK QTKVTSVGNP TIKPHSVPSH TLPSHPVTPS SKHADSKPAP LTPAYHTLPH PSHHGTPHTT INWGPLEPPK TPKPW

Highest antigen sequence identity to the following orthologs: Mouse - 94%, Rat - 95%.

Target Information

The serine/threonine kinase, p21 activated kinase (PAK), is a downstream effector of the small GTPases Cdc42 and Rac. PAK associates with Nck, the p85 and p110 subunits of PI3-kinase and PIX (PAK-interacting exchange factor) in a focal complex. The binding of PIX is necessary for the localization and activation of PAK in the Cdc42 to Rac signaling pathway, and this binding occurs through the high affinity of the N-terminal SH3 domain of PIX for a conserved proline rich PAK sequence. PIX exists as two isoforms, alpha and beta, and both are highly expressed in heart, muscle and thymus tissues of human and rat. alphaPIX is phosphorylated via PDGF and EphB2 receptor signaling pathways or through association with PI3-kinase. The alphaPIX isoform predominantly acts as a guanine nucleotide exchange factor (GEF) on Rac, which may mediate lamellipodia formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.