Thermo Fisher Scientific SMAD1 Monoclonal Antibody (1D3)
SMAD1 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC/IF, ELISA에 사용 가능. 인간 반응성, 비결합형 액상 형태, PBS 저장. BMP 신호전달 연구 및 세포 성장·분화 관련 연구에 적합.
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Thermo Fisher Scientific SMAD1 Monoclonal Antibody (1D3)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 3 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1D3 |
| Immunogen | SMAD1 (NP_005891, 1–110 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
MNVTSLFSFT SPAVKRLLGW KQGDEEEKWA EKAVDALVKK LKKKKGAMEE LEKALSCPGQ PSNCVTIPRS LDGRLQVSHR KGLPHVIYCR VWRWPDLQSH HELKPLECCE
Target Information
The protein encoded by this gene belongs to the SMAD family, which is homologous to the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma.
SMAD proteins act as signal transducers and transcriptional modulators mediating multiple signaling pathways.
SMAD1 mediates bone morphogenetic protein (BMP) signaling involved in cell growth, apoptosis, morphogenesis, development, and immune responses.
Upon BMP ligand binding, SMAD1 is phosphorylated and activated by BMP receptor kinase, forming a complex with SMAD4 for transcriptional regulation.
It is a target for SMAD-specific E3 ubiquitin ligases (SMURF1, SMURF2) and undergoes ubiquitination and proteasome-mediated degradation.
Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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