Thermo Fisher Scientific JMJD1A Polyclonal Antibody
Human KDM3A 인식 토끼 폴리클로날 항체로 Western blot 및 ICC/IF에 적합. 보존제 무첨가, PBS 용액 형태로 제공. 단기 4°C, 장기 -20°C 보관 권장. 히스톤 탈메틸화 효소 연구용으로 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:1,500–1:50,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the C-terminus of Human KDM3A (UniProt ID: Q9Y4C1-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2788434 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human KDM3A.
Target Information
JMJD1A is a histone demethylase that specifically demethylates Lys-9 of histone H3, playing a central role in the histone code.
It preferentially demethylates mono- and dimethylated H3 Lys-9 residues, with higher activity toward dimethylated forms, and minimal activity on trimethylated H3 Lys-9.
Demethylation generates formaldehyde and succinate. JMJD1A participates in hormone-dependent transcriptional activation by recruitment to androgen receptor target genes, leading to H3 Lys-9 demethylation and transcriptional activation.
It is also involved in spermatogenesis by regulating expression of genes such as PRM1 and TMP1, and contributes to obesity resistance through regulation of metabolic genes including PPARA and UCP1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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