Thermo Fisher Scientific Mouse IgM Isotype Control

Applications

Tested Dilution

Flow Cytometry (Flow)

Neat

Control (Ctrl)

Assay-Dependent

Product Specifications

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Isotype Control

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2550643

Product Specific Information

PA5-33228 may be used as an isotype matched negative control to determine the level of non-specific binding of IgM monoclonal antibodies in human leucocytes. The antibody does not recognize human antigen. This antibody recognizes a rat cell surface marker and therefore cannot be used as a negative control in the species.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.