Thermo Fisher Scientific PGP9.5 Monoclonal Antibody (31A3)
PGP9.5 단백질을 인식하는 Mouse Monoclonal Antibody (Clone 31A3). 다양한 종(Human, Mouse, Rat, Rabbit)에 반응하며, WB, IHC, ICC, ELISA에 사용 가능. 신경세포 및 신경조직 연구에 적합하며, Protein A로 정제된 고순도 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | 13 publications |
| Immunohistochemistry (IHC) | – | 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:400 | – |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 2 publications |
| ELISA | 1:500–1:2,000 | – |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rabbit, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 31A3 |
| Immunogen | Native protein from brain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_935198 |
Product Specific Information
- Does not react with guinea pig.
- Suitable for paraffin-embedded tissue sections. Recommended fixation: 95% ethanol / 5% acetic acid for 2–3 hours before embedding.
- Specimens not fixed in acetic acid/alcohol may require pretreatment using microwave-citrate buffer method.
- Mouse anti-Human Protein Gene Product 9.5 antibody (clone 31A3) recognizes PGP9.5, a ubiquitin hydrolase widely expressed in neuronal tissues, representing 1–2% of total soluble brain proteins.
Target Information
PGP9.5 (Protein Gene Product 9.5, also known as UCH-L1 or PARK5) is a neuron-specific protein, distinct from neuron-specific enolase.
It has a molecular weight of 27 kDa and belongs to the ubiquitin carboxyl-terminal hydrolase family (peptidase family C12).
PGP9.5 exhibits ubiquitin hydrolase and ligase activities, hydrolyzing small C-terminal adducts of ubiquitin to generate monomers.
It is present in neurons and nerve fibers throughout the central and peripheral nervous systems, as well as in neuroendocrine cells, renal tubules, spermatogonia, Leydig cells, ova, and corpus luteum cells.
Overexpression is linked to non-small cell lung cancer, while reduced expression is associated with Huntington’s and Alzheimer’s diseases.
PGP9.5 serves as a useful neuronal marker in neurodegenerative disorder studies, including Parkinson’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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