Thermo Fisher Scientific Phospho-FOXO3A (Ser253) Polyclonal Antibody
FOXO3A 단백질의 Ser253 인산화 부위를 인식하는 폴리클로날 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. 인간, 마우스, 랫트 반응성. 액상 형태, 1 mg/mL 농도, -20°C 보관. 연구용 전용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | View 2 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | – |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of serine 253 (A‑V‑S(p)‑M‑D) derived from Human FKHRL1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554186 |
Product Specific Information
- Suggested positive control for Western blot: NIH‑3T3 cells
- Suggested positive control for IHC: human breast carcinoma
- Suggested positive control for ICC/IF: Hela cells
Target Information
USP48 is a protein containing domains that associate it with the peptidase family C19, also known as family 2 of ubiquitin carboxyl-terminal hydrolases.
Family members function as deubiquitinating enzymes, recognizing and hydrolyzing the peptide bond at the C-terminal glycine of ubiquitin.
Enzymes in peptidase family C19 are involved in the processing of poly‑ubiquitin precursors as well as that of ubiquitinated proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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