Thermo Fisher Scientific IL-2 Polyclonal Antibody

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant human IL-2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223480

Product Specific Information

P600 targets IL-2 in ELISA applications and shows reactivity with Human samples.

The P600 immunogen is recombinant human IL-2.

P600 detects IL-2 which has a predicted molecular weight of approximately 15 kDa.

The P600 IL2 polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M600B (biotinylated conjugate of clone BG5). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody P600 and biotinylated antibody M600B (clone BG5) have successfully been used in combination with recombinant IL2 protein SIL2 in ELISA applications.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.