Thermo Fisher Scientific TRIP230 Monoclonal Antibody (10G5)
TRIP230 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, ICC/IF, IP에 사용 가능. Human 시료 반응성. Protein G 정제, PBS buffer, 1 mg/mL 농도. 연구용으로 MCF7, HBL100, T24 세포에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 2 publications |
| Immunocytochemistry (ICC/IF) | Assay-dependent | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10G5 |
| Immunogen | Amino acids 1099–1372 of Trip230 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term; for long-term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2209356 |
Product Specific Information
Recommended positive controls: MCF7, HBL100, T24.
Target Information
The Trip230 gene encodes a protein of 1,978 amino acids with an estimated molecular mass of 230 kDa.
It is located on chromosome 14q31, a region associated with genetic alterations and thyroid hormone response abnormalities.
Trip230 interacts with Rb and thyroid hormone receptor (TR) through distinct domains.
- Complex formation with Rb occurs in a hormone-independent manner.
- Complex formation with TR is thyroid hormone-dependent.
After T3 treatment, Trip230 is phosphorylated and translocated to the nucleus, suggesting phosphorylation is important for its nuclear localization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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