Thermo Fisher Scientific PREB Polyclonal Antibody
PREB 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 고순도 PBS 버퍼에 보존됩니다. 인간 PREB 단백질에 특이적으로 반응합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:500 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human PREB. Recombinant protein control fragment (Product #RP-90970) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645928 |
Product Specific Information
Immunogen sequence:
LRFQAHQQQG NKAEKAGSKE QGPRQRKGAA PAEKKCGAET QHEGLELRVE NLQAVQTDFS SDPLQKVVCF NHDNTLLATG GTDGYVRVWK VPSLEKVLEF KAHEGEIEDL ALGPDGKLVT VGRDLKASVW QKDQLVTQLH WQENGPT
Sequence identity to orthologs:
- Mouse: 84%
- Rat: 87%
Target Information
Prolactin (PRL) expression in the pituitary is limited to specific cells. Pit-1 is a pituitary-specific transcription factor that plays an important role in PRL expression during development and in mature organisms. The PRL promoter contains numerous Pit-1 binding sites involved in basal and kinase-mediated gene expression.
PREB (prolactin regulatory element binding protein) regulates PRL gene expression through the 1P site without a discernable DNA-binding motif. It is encoded by a single-copy gene in both mice and humans and accumulates in pituitary cell nuclei. PREB functions as a novel transcription factor assisting PRL expression alone or with Pit-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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