Thermo Fisher Scientific Esrp-1 Monoclonal Antibody (27H12.F3.H5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

ELISA (ELISA)

1:750,000

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

27H12.F3.H5

Immunogen

Anti-Esrp-1 was produced by repeated immunizations of full length recombinant mouse Esrp-1 fusion protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ESRP1,uniProtId:Q3US41-1,ncbiNodeId:10090,antigenRange:1-680,antigenLength:680,antigenImageFileName:210-301-B89_ESRP1_Q3US41-1_House_mouse.svg,antigenImageFileNamePDP:210-301-B89_ESRP1_Q3US41-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This antibody reacts with mouse Esrp-1 protein. A BLAST analysis of the immunizing protein sequence shows 100% homology with Esrp-1 from mouse and a 91% sequence homology with Esrp-1 from human, pig, rat, opossum, horse, cattle, panda, dog, and chimpanzee. The binding epitope of this monoclonal antibody has not been mapped.

Target Information

RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5` UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.