Thermo Fisher Scientific DCLK1 Polyclonal Antibody
Rabbit polyclonal antibody against human DCLK1 for WB and ICC/IF applications. Recognizes recombinant human DCAMKL1 antigen. Supplied as liquid form, unconjugated, purified by antigen affinity chromatography. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.25 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human DCAMKL1. Recombinant protein control fragment (Product # RP-90899) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2662055 |
Product Specific Information
Immunogen sequence:
TSSVNGTPGSQ LSTPRSGKSP SPSPTSPGSL RKQRSSQHGG SSTSLASTKV CSSMDENDGP GEEVSEEGFQ IPATITERYK VGRTIGDGNF AVVKECVERS TAREYALKII K
Highest antigen sequence identity to the following orthologs:
- Mouse: 96%
- Rat: 96%
Target Information
DCAMKL1 (DCLK1) — also known as doublecortin-like kinase 1 — is a serine/threonine kinase containing two N-terminal doublecortin domains that bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain homologous to Ca²⁺/calmodulin-dependent protein kinase, and a serine/proline-rich domain mediating multiple protein-protein interactions.
DCAMKL1 is a microtubule-associated kinase capable of autophosphorylation and exhibits microtubule-polymerizing activity independent of its kinase activity. It participates in neuronal migration, retrograde transport, neuronal apoptosis, and neurogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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