Thermo Fisher Scientific Pol Iota Polyclonal Antibody
인간 DNA polymerase iota에 특이적인 Rabbit Polyclonal Antibody로, Western blot 및 Immunoprecipitation에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 1 mg/mL 농도입니다. DNA 수선 및 translesion 합성 연구용에 적합합니다.
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Thermo Fisher Scientific Pol Iota Polyclonal Antibody
Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 640 and 690 of Human DNA polymerase iota |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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