Thermo Fisher Scientific GLI2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-2.5 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-10 µg/mL

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from a region between residues 272 - 321 of human Gli2. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GLI2,uniProtId:P10070-5,ncbiNodeId:9606,antigenRange:272-321,antigenLength:1586,antigenImageFileName:PA1-30042_GLI2_P10070-5_Rabbit.svg,antigenImageFileNamePDP:PA1-30042_GLI2_P10070-5_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2294766

Product Specific Information

Predicted molecular weight of 133 kDa.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.