Thermo Fisher Scientific TRAF5 Monoclonal Antibody (55A219)
TRAF5 단백질을 인식하는 Mouse IgG 단일클론 항체로, Western blot에 최적화됨. Human 시료에 반응하며, Protein G 정제 및 PBS/BSA 버퍼로 제공. 세포 신호전달 연구 및 TNF 수용체 관련 단백질 분석에 적합.
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Applications
- Western Blot (WB)
Tested Dilution: 2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 55A219 |
| Immunogen | Fusion protein corresponding to amino acids 77–186 of human TRAF5 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2209719 |
Product Specific Information
Suggested positive control: HeLa whole cell extract, antigen standard for TRAF5 (transient overexpression lysate).
Target Information
Tumor necrosis factor (TNF) induced signaling occurs via TNF receptor (TNFR) interactions with adaptor proteins such as TRAFs. TRAFs are cytoplasmic adapter proteins mediating signal transduction from TNF receptor superfamily members (e.g., RANK, CD30, CD40) and interleukin-1 receptor. The C-terminal region of TRAFs is essential for self-association and receptor interaction. Six TRAFs (TRAF1–TRAF6) have been identified. TRAF5, a 558-amino acid protein, is involved in NF-κB and JNK/SAPK activation by TNFR family members including CD27, CD30, CD40, and lymphotoxin-β receptor. TRAF5 gene disruption leads to defects in CD40-CD27 mediated lymphocyte activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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