Thermo Fisher Scientific Bi-1 Monoclonal Antibody (20F430)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5-10 µg/mL

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

20F430

Immunogen

A synthetic peptide made to the N-terminal portion of the human Bax inhibitor 1 protein (between amino acids 1-40) UniProt P55061. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Bi-1,uniProtId:P55061-1,ncbiNodeId:9606,antigenRange:1-40,antigenLength:237,antigenImageFileName:MA1-41108_Bi-1_P55061-1_House_mouse.svg,antigenImageFileNamePDP:MA1-41108_Bi-1_P55061-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.2% gelatin

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2204052

Product Specific Information

Epitope mapping indicates that the antibody recognizes an epitope in the N-terminal region of Bi-1.

Target Information

BI-1 (Bax Inhibitor 1) is in anti-apoptotic protein that has been linked to protection from apoptosis induced endoplasmic reticulum (ER) stress (reviewed in Bailley-Maitre et al, 2007). BI-1 contains several transmembrane domains, localizes to ER membranes, and has cytoprotective functions that are conserved in both animals and plants. BI-1 suppresses apoptosis induced by ectopic expression of the proapoptotic protein Bax as well as other types of stimuli. Cells from BI-1 knockout (KO) mice are hypersensitivite to apoptosis induced by ER stress-causing chemical agents (thapsigargin, tunicamycin, and brefeldin A) or by ischemia-reperfusion (IR) injury. Conversely, overexpression of BI-1 protects against apoptosis induced by ER stress and IR. The mechanism by which BI-1 protects cells from ER-stress induced apoptosis remains to be fully elucidated, itis thought to involve regulation of Ca2+ handling by the ER.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.