Thermo Fisher Scientific Bi-1 Monoclonal Antibody (20F430)
Bi-1 단백질을 인식하는 mouse monoclonal antibody로, Western blot 및 IHC에 사용 가능. Human, Mouse, Rat 반응성. Protein G 정제 및 PBS buffer 보존. ER stress 관련 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | Assay-dependent | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 20F430 |
| Immunogen | A synthetic peptide made to the N-terminal portion of the human Bax inhibitor 1 protein (amino acids 1–40), UniProt P55061 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.2% gelatin |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2204052 |
Product Specific Information
Epitope mapping indicates that the antibody recognizes an epitope in the N-terminal region of Bi-1.
Target Information
BI-1 (Bax Inhibitor 1) is an anti-apoptotic protein associated with protection from apoptosis induced by endoplasmic reticulum (ER) stress. BI-1 contains multiple transmembrane domains, localizes to ER membranes, and exhibits conserved cytoprotective functions in animals and plants.
It suppresses apoptosis triggered by the proapoptotic protein Bax and other stimuli. Cells from BI-1 knockout mice are hypersensitive to apoptosis induced by ER stress agents (thapsigargin, tunicamycin, brefeldin A) or ischemia-reperfusion injury. Conversely, BI-1 overexpression provides protection against such stress-induced apoptosis, potentially through regulation of ER Ca²⁺ handling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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