Takara 10-minute, quantitative lentiviral titer test—Lenti-X GoStix Plus

Lenti-X GoStix Plus provide a rapid, simple, and effective method for instantly quantifying lentivirus in packaging cell supernatants. The test involves applying 20 µl of supernatant to a GoStix cassette and waiting 10 minutes for the appearance of bands that indicate the presence of lentiviral p24. Whereas our original GoStix provided a qualitative output, results from Lenti-X GoStix Plus can be analyzed with a free smartphone app, which quantifies lentivirus titer by comparing the band intensities.

Lenti-X GoStix Plus provide a rapid, simple, and effective method for instantly quantifying lentivirus in packaging cell supernatants. The test involves applying 20 µl of supernatant to a GoStix cassette and waiting 10 minutes for the appearance of bands that indicate the presence of lentiviral p24. Whereas our original GoStix provided a qualitative output, results from Lenti-X GoStix Plus can be analyzed with a free smartphone app, which quantifies lentivirus titer by comparing the band intensities.

The quantitative output from the Lenti-X GoStix App (referred to as a GoStix Value, or GV) can be used to compare virus amounts between different preparations in a manner similar to, but far easier and quicker than, an ELISA, and allows for calculation of actual IFU/ml using a reference virus. Visit our FAQs page for information on how to download and use the smartphone app.

By enabling quick and easy lentivirus quantitation, Lenti-X GoStix Plus allow you to minimize variability in your transduction protocol and achieve consistent performance for each experiment.

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