Thermo Fisher Scientific TEM7 Monoclonal Antibody (197C193 (IM193))
TEM7 단클론 항체로 인간, 마우스, 랫트에서 반응하며 다양한 면역실험(WB, IHC, ICC 등)에 사용 가능. 단백질 G로 정제된 액상형 비결합 항체. 종양 혈관 내피세포 특이적 TEM7 단백질 연구에 적합. 연구용으로만 사용.
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Thermo Fisher Scientific TEM7 Monoclonal Antibody (197C193 (IM193))
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–3 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 2–5 µg/mL |
| Immunohistochemistry (Frozen) (IHC-F) | 2–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:10–1:500 |
| Immunoprecipitation (IP) | 1:10–1:500 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 197C193 (IM193) |
| Immunogen | Synthetic peptide corresponding to residues 409–425 of human TEM7 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at –20°C (avoid freeze/thaw cycles) |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2166233 |
Product Specific Information
Suggested positive control: antigen standard for PLXDC1 (transient overexpression lysate), HCT-116.
Target Information
Using SAGE (Serial Analysis of Gene Expression) technology, St. Croix et al. identified 46 genes with elevated expression in tumor-associated endothelium. Nine of these genes, named Tumor Endothelial Markers (TEMs, TEM1–9), are prominently expressed only in tumor endothelial cells and are absent or barely detectable in normal endothelial cells.
TEM7 (Tumor Endothelial Marker 7) transcripts are specifically expressed in the endothelium of colorectal, lung, pancreas, breast, and brain cancers, both primary and metastatic. Other family members (TEM1, 3, 4, 5, 8, and 9) show similar expression patterns in lung and brain tumors and liver metastasis.
These genes, differentially expressed during angiogenesis, provide valuable resources for basic and clinical studies of human angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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