Thermo Fisher Scientific MMP9 Monoclonal Antibody (IIA5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 9 publications 9 publications

Immunohistochemistry (IHC)

-

View 11 publications 11 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

-

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

Immunoprecipitation (IP)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Published species

Dog, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

IIA5

Immunogen

Human native 92kDa MMP-9 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MMP-9,uniProtId:P14780-1,ncbiNodeId:9606,antigenRange:1-707,antigenLength:707,antigenImageFileName:MA5-14228_MMP-9_P14780-1_House_mouse.svg,antigenImageFileNamePDP:MA5-14228_MMP-9_P14780-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11004645

Product Specific Information

MA5-14228 targets MMP-9 (92kDa Collagenase IV) in IHC, IP, and WB applications and shows reactivity with Human and Rat samples.

The MA5-14228 immunogen is human native 92kDa MMP-9.

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.