Thermo Fisher Scientific LIMK2 Polyclonal Antibody, MaxPab

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LIMK2 (NP_001026971.1, 1 a.a. approximately 686 a.a) full-length human protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MGSYLSVPAY FTSRDLFRCS ECQDSLTNWY YEKDGKLYCP KDYWGKFGEF CHGCSLLMTG PFMVAGEFKY HPECFACMSC KVIIEDGDAY ALVQHATLYC GKCHNEVVLA PMFERLSTES VQEQLPYSVT LISMPATTEG RRGFSVSVES ACSNYATTVQ VKEVNRMHIS PNNRNAIHPG DRILEINGTP VRTLRVEEVE DAISQTSQTL QLLIEHDPVS QRLDQLRLEA RLAPHMQNAG HPHALSTLDT KENLEGTLRR RSLRRSNSIS KSPGPSSPKE PLLFSRDISR SESLRCSSSY SQQIFRPCDL IHGEVLGKGF FGQAIKVTHK ATGKVMVMKE LIRCDEETQK TFLTEVKVMR SLDHPNVLKF IGVLYKDKKL NLLTEYIEGG TLKDFLRSMD PFPWQQKVRF AKGIASGMAY LHSMCIIHRD LNSHNCLIKL DKTVVVADFG LSRLIVEERK RAPMEKATTK KRTLRKNDRK KRYTVVGNPY WMAPEMLNGK SYDETVDIFS FGIVLCEIIG QVYADPDCLP RTLDFGLNVK LFWEKFVPTD CPPAFFPLAA ICCRLEPESR APPGAAGEGP GCADDEGPVR RQGKVTIKYD PKELRKHLNL EEWILEQLTR LYDCQEEEIS ELEIDVDELL DMESDDAWAS RVKELLVDCY KPTEAFISGL LDKIRAMQKL STPQKK

Target Information

LIMK2 is a serine/threonine kinase involved in regulation of actin cytoskeletal changes via phosphorylation of cofilin. LIMK2 has also been implicated in brain development. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for LIMK2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.