Thermo Fisher Scientific N-cadherin Polyclonal Antibody
Thermo Fisher Scientific의 N-cadherin Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하며 WB, IHC, ELISA에 사용 가능. Rabbit IgG 기반의 액상 항체로 0.5 mg/mL 농도, PBS/glycerol buffer에 보존. 세포막 단백질 검출에 적합하며 연구용으로만 사용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide (amino acids 807–906 of human N-Cadherin, NP_0017832) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS (pH 7.3) with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804146 |
Product Specific Information
- Positive Samples: 293T, Mouse liver, Mouse brain, Mouse heart, Rat liver
- Cellular Location: Cell membrane, Single-pass type I membrane protein
Immunogen sequence:
RRMDERPIHA EPQYPVRSAA PHPGDIGDFI NEGLKAADND PTAPPYDSLL VFDYEGSGST AGSLSSLNSS SSGGEQDYDY LNDWGPRFKK LADMYGGGDD
Target Information
N-cadherin is a 140 kDa transmembrane protein that mediates calcium-dependent intercellular adhesion. It plays a key role in morphogenetic movements during development and regulates cell surface adhesion through homotypic interactions. The function of N-cadherin depends on its association with the actin-cytoskeleton via catenin proteins, regulated by phosphorylation of beta-catenin. Structurally, it contains five extracellular cadherin repeats, a transmembrane region, and a conserved cytoplasmic tail. It is essential for gastrulation and establishment of left-right asymmetry.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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