Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Brilliant Ultra Violet 615, eBioscience

Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Brilliant Ultra Violet 615, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 563
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 605
• eFluor 450
• eFluor 506
• eFluor 660
• FITC
• Functional Grade
• NovaFluor Blue 510
• NovaFluor Blue 585
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It has potent immunosuppressive properties and has been effective in treating renal, heart, and liver allograft rejection. CD3 is expressed by thymocytes and all mature T cells. Crosslinking of TCR initiates intracellular signaling leading to activation and proliferation.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on human peripheral blood cells at 5 µL (0.125 µg)/test.

Brilliant Ultra Violet™ 615 (BUV615) emits at 615 nm and is intended for use on cytometers equipped with a 355 nm ultraviolet laser. Ensure instrument compatibility.

Staining Recommendations:

• Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize polymer dye interactions.
• Protect from light due to photo-induced oxidation sensitivity.
• Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark.

Excitation: 350 nm
Emission: 615 nm
Laser: Ultraviolet Laser

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, encoded on chromosome 11. CD3 is expressed on T lymphocytes and thymocytes, but not on B or NK cells. Defects in CD3 genes are linked to CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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