Thermo Fisher Scientific ELK1 Recombinant Rabbit Monoclonal Antibody (ARC0339)
ELK1 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, ELISA, IP에 적합합니다. HEK293 세포에서 발현된 IgG 형 항체이며, 높은 특이성과 재현성을 제공합니다. 연구용으로 사용되며, MAP kinase 신호 경로 연구에 활용됩니다.
- 카탈로그번호
- MA535227
- 판매단위
- pk
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Thermo Fisher Scientific ELK1 Recombinant Rabbit Monoclonal Antibody (ARC0339)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0339 |
| Immunogen | Synthetic peptide corresponding to amino acids 329–428 of human ELK1 (NP_001107595.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849131 |
Product Specific Information
Immunogen sequence:
GGPGPERTPG SGSGSGLQAP GPALTPSLLP THTLTPVLLT PSSLPPSIHF WSTLSPIAPR SPAKLSFQFP SSGSAQVHIP SISVDGLSTP VVLSPGPQKP
Target Information
ELK1 is a component of the ternary complex that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. ELK1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus. Phosphorylation at these sites, particularly Ser383, is critical for transcriptional activation by ELK1. ELK1 appears to be a direct target of activated MAP kinase. Biochemical studies indicate that ELK1 is a good substrate for MAP kinase, the kinetics of ELK1 phosphorylation and activation correlate with MAP kinase activity, and interfering mutants of MAP kinase block ELK1 activation in vivo. ELK1 is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for ELK1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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