Thermo Fisher Scientific HEF1 Monoclonal Antibody (2G9)

Thermo Fisher Scientific HEF1 Monoclonal Antibody (2G9)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at -20°C prior to opening.
• Aliquot contents and freeze at -20°C or below for extended storage.
• Avoid cycles of freezing and thawing.
• Centrifuge product if not completely clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

This antibody is directed against human HEF1 protein and purified from tissue culture supernatant by chromatography.
Reactivity occurs against human, mouse, and rat forms of the protein.
Reactivity against multiple isoforms is expected.
Specificity was determined by partial epitope mapping.

Target Information

HEF1 (Human Enhancer of Filamentation 1) is a multifunctional protein involved in integrin-based signaling that regulates cell motility, growth, apoptosis, and oncogenic transformation.
It belongs to the Cas family of docking proteins, substrates of focal adhesion kinase (FAK) and Src family tyrosine kinases—key targets for drug development.
HEF1 protein promotes expression of genes linked to motility and invasiveness, such as metalloproteinases, MLCK, p160ROCK, and ErbB1.
Overproduction of HEF1 mediates apoptosis in epithelial-derived cell lines (e.g., MCF7, HeLa).
Overexpression of BCAR1 (p130Cas), a related protein, has been associated with tamoxifen resistance, underscoring the role of this protein family in cancer prognosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.