Thermo Fisher Scientific IL-2 Monoclonal Antibody (AB12-3G4), Functional Grade, eBioscience

Applications

Neutralization (Neu)

• Assay-Dependent

Functional Assay

• Assay-Dependent
• Refer to publication for details.

Product Specifications

Product Specific Information

Description:
The AB12-3G4 monoclonal antibody reacts with and inhibits the bioactivity of human IL-2. IL-2 is a 15 kDa cytokine expressed by B cells, T cells, and dendritic cells. It signals via a heterotrimeric receptor (CD25) and activates the Jak/STAT pathway. IL-2 is critical for T cell proliferation, activation, and differentiation, particularly in the development of CD4+CD25+ regulatory T cells.

Applications Reported:
This antibody inhibits the bioactivity of human IL-2.

Applications Tested:
The ND50 of AB12-3G4, as determined by the neutralization of Human IL-2-induced proliferation of the CTLL-2 cell line, is ≤60 ng/mL in the presence of 10 ng/mL Human IL-2 Recombinant Protein. The neutralization dose may vary depending on cell type, assay method, and cytokine concentration.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90%, as determined by SDS-PAGE.

Endotoxin Level:
<0.001 ng/µg antibody, as determined by LAL assay.

Aggregation:
<10%, as determined by HPLC.

Target Information

Interleukin 2 (IL-2) is an immunomodulatory cytokine critical for the proliferation of activated T cells and the differentiation of B cells, natural killer cells, monocytes, and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is shared with IL-4 and IL-7. The IL-2 gene exhibits monoallelic expression in mature thymocytes, representing a unique regulatory mechanism. Disruption of an IL-2-like gene in mice results in an ulcerative colitis-like disease, demonstrating its essential role in immune response regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.