Invitrogen™

Poly(A)Purist™ MAG Kit

Ambion™ Poly(A)Purist™ MAG Kit (patent pending) uses magnetic technology for the selection of mRNA that typically requires only a single자세히 알아보기

Ambion™ Poly(A)Purist™ MAG Kit (patent pending) uses magnetic technology for the selection of mRNA that typically requires only a single round of selection yet yields RNA that is more highly enriched and devoid of rRNA than other procedures. Each kit contains sufficient reagents for the isolation of mRNA from up to 8 mg of total RNA.

• Highest recovery of mRNA of any available kit
• High stringency for lowering residual rRNA content
• Single round of selection sufficient for most applications

The superior performance of Poly(A)Purist™ MAG Kit is clearly demonstrated by a comparison of the mRNA recovered using 4 other kits (see Figure). The kit uses batch binding of RNA to premeasured aliquots of oligo(dT) cellulose to circumvent the problems of slow flow rates and clogged columns often experienced during conventional, gravity-driven chromatography. The magnetic bead–based selection employed in the delivers increased processing speed, ease of handling, and scalability. The entire selection procedure can be completed in as little as 45 minutes (see Figure).

Accessory Products:
A magnetic stand is required for use this kit. They are available in several formats, including the Single Place Magnetic Stand (Cat. No. AM10026) and the 6 Tube Magnetic Stand (Cat. No. AM10055).

User Guide Alert (July 2023): Step 3 of the Preparation of Oligo (dT) MagBeads section (page 7) of the User Guide is misleading. Please follow this instruction for Step 3: add an equivalent mass of Oligo (dT) Magbeads into the selected empty tube and then continue with Oligo (dT) Magbeads preparation according to steps 4-9.

사양

형식Kit

Isolation TechnologyMagnetic Bead, Oligo dT Affinity

샘플 종류Total RNA

최종 제품 유형mRNA

고처리량 호환성Not High-throughput Compatible (Manual)

용도(애플리케이션)Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), Microinjection, Nucleic Acid Labeling, In Vitro Translation, cDNA Library Construction, Northern Blotting, Nuclease Protection Assays

제품라인Ambion™, Poly(A)Purist™

제품 유형MAG Kit

수량1 kit

배송 조건Room Temperature

TargetmRNA

테스트 시간45 min.

Unit SizeEach