Invitrogen™

RiboPure™ RNA Purification Kit, yeast

For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression자세히 알아보기

For the isolation of extremely high-quality RNA from yeast without the use of enzymatic pretreatments that may alter gene expression profiles. Each Ambion™ kit includes sufficient reagents for 50 purifications each from 107 to 108 yeast cells.

• Highest yields of total RNA available
• No enzymatic pretreatment required
• Includes DNA-_free_™ Reagents

It has traditionally been difficult to isolate high-quality RNA from yeast because of problems disrupting the cells. Many protocols use enzymatic pretreatments that may affect gene expression patterns. The RiboPure™-Yeast RNA Isolation Kit uses no enzymatic pretreatments and thus does not alter expression profiles. It combines a simple and efficient glass bead/one step phenol–mediated disruption using Ambion`s Vortex Bead Beater Adapter (see Accessory Products) followed by a glass fiber filter– based RNA purification step. After DNase treatment with the included DNA-free_™ Reagents, the resulting RNA is exceptionally pure containing little to no genomic DNA (less than 0.05%). The kit has been used extensively at Ambion to isolate high-quality RNA from _Saccharomyces cerevisiae, Schizosaccharomyces pombe, Pichia pastoris, and Candida albicans (see Figure). The kit provides the highest yield of any method tested from each of these species (see Figure).

Accessory Products:
The Kit requires the use of the Vortex Adapter (Cat. No. AM10024), which turns a standard laboratory vortex into a bead beater. This adapter fits the Vortex Genie™ 2 and holds 12 tubes.

사양

Isolation TechnologySpin Column (Glass Fiber Filter)

정제 시간1.5 hr. (Including DNase treatment)

용리량50–100 μL

샘플 종류Yeast

최종 제품 유형Total RNA

고처리량 호환성Not High-throughput Compatible (Manual)

용도(애플리케이션)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling

수량50 Preps

배송 조건Box 1: Dry Ice
Box 2: Room Temperature

시작 물질 양Up to 108 cells

수율Up to 300 μg

Unit SizeEach